DNA

Part:BBa_K4727108

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-09-15)


sgDNA AmpC 148

This guide DNA has been designed to work with dCas9 to target AMR related genes in Acinetobacter baumannii . The guide has been designed to bins 148bp downstream the TSS

blaADC, also called blaAmpC, is a class C β-lactamase, i.e. a cephalosporinase encoded by the chromosome in A. baumannii. It involves resistance to Ampicillin, especially when it is overexpressed due to the insertion of short transposon sequences, which are stronger promoters than the intrinsic one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None